diff --git a/pyproject.toml b/pyproject.toml
index 2850ade6988c..359da460e249 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -106,7 +106,7 @@ select = [
     "PYI044", # `from __future__ import annotations` has no effect in stub files, since type checkers automatically treat stubs as having those semantics
     "PYI055", # Multiple `type[T]` usages in a union. Combine them into one, e.g., `type[{union_str}]`.
     "PYI058", # Use `{return_type}` as the return value for simple `{method}` methods
-    # "PYI059", # TODO: Add when dropping Python 3.9 support
+    "PYI059", # Checks for classes inheriting from typing.Generic[] where Generic[] is not the last base class in the bases tuple
     "PYI061", # Use `None` rather than `Literal[None]`
     "PYI062", # Duplicate literal member `{}`
     "PYI064", # `Final[Literal[{literal}]]` can be replaced with a bare Final
diff --git a/stdlib/asyncio/queues.pyi b/stdlib/asyncio/queues.pyi
index 2fa2226d0e6a..de19fa236ad3 100644
--- a/stdlib/asyncio/queues.pyi
+++ b/stdlib/asyncio/queues.pyi
@@ -4,10 +4,7 @@ from asyncio.events import AbstractEventLoop
 from types import GenericAlias
 from typing import Any, Generic, TypeVar
 
-if sys.version_info >= (3, 10):
-    from .mixins import _LoopBoundMixin
-else:
-    _LoopBoundMixin = object
+from .mixins import _LoopBoundMixin
 
 class QueueEmpty(Exception): ...
 class QueueFull(Exception): ...
@@ -24,9 +21,7 @@ _T = TypeVar("_T")
 if sys.version_info >= (3, 13):
     class QueueShutDown(Exception): ...
 
-# If Generic[_T] is last and _LoopBoundMixin is object, pyright is unhappy.
-# We can remove the noqa pragma when dropping 3.9 support.
-class Queue(Generic[_T], _LoopBoundMixin):  # noqa: Y059
+class Queue(_LoopBoundMixin, Generic[_T]):
     if sys.version_info >= (3, 10):
         def __init__(self, maxsize: int = 0) -> None: ...
     else: